PREPARATION OF A NEW AZO COMPOUND (HAZM) USED FOR ANALYTICAL SPECTROPHOTOMETRIC DETERMINATION OF GLUCOSE IN BLOOD AND SALIVA

Abstract

Because health resources are limited, low-income conditions demand affordable technological development. The design and analysis is a low-cost colorimetric method for non-invasive diabetic diagnostic monitoring by glucose detection of the saliva and blood discussed, where a new compound (HAZM) was prepared through a reaction 4-aminoantipyrine with 1-phenyl-2-methyl-5-pyrazolon; FTIR, 1HNMR, and 13CNMR diagnosed for this synthetic compound. Samples have been processed and analyzed using the production equipment using glucose oxidase (GO) and peroxidase (PO) enzymes reaction with (HAZM); give the method the ability to calculate glucose level in less than 15 seconds accurately. The slope of the calibration curve is equal to 0.0026, ε = 468.26, and Sandel sensitive equals 0.384 mg/dL, to quantify the glucose salivary concentrations and blood (5-500 mg/dL) at 423 nm. Achieve a detection limit of 0.421 mg/dL where 5 % CV to (n = 3). Because HAZM tests performed according to a kit method, which reflects the mean kit values (101.35 ± 3.647 mg/dL) and the results of HAZM (104.41 ± 4.951 mg/dL) with 3.1 mg/dL positive bias in blood. Comparing the mean kit and HAZM glucose levels in saliva, 99.48 ± 1.26, and 100.97 ± 2.38 mg/dL, respectively, shows 1.5 mg/dL positive bias for the kit process. Results from the use of this method for measuring glucose in blood and saliva samples indicate that it is effective, as it offers accurate, and its cheap results compared to other commercial techniques.