Abstract
In this paper, a cartilage acellular-matrix (CAM) is chosen as a biomaterial for an effective antiadhesive barrier to apply between injured tissue and healthy tissues or organs. CAM is cross-linked using glutaraldehyde to create a cross-linked CAM (Cx-CAM) film. Cx-CAM has higher elastic modulus and toughness and more hydrophobic surface properties than CAM before cross-linking. Small intestinal submucosa (SIS), cross-linked SIS (Cx-SIS) as a negative control, and Seprafilm as a positive control are used in an experiment as adhesion barriers. Human umbilical vein endothelial cells (HUVECs) on SIS, Cx-SIS, or in a culture plate get attached and effectively proliferate for 7 days, but Cx-CAM and Seprafilm allow for little or no attachment and proliferation of HUVECs, thus manifesting antiadhesive and antiproliferative effects. In animals with surgical damage to the peritoneal wall and cecum, Cx-CAM and Seprafilm afford little adhesion and negligible inflammation after seven days, as confirmed by hematoxylin and eosin staining and macrophage staining, in contrast to an untreated-injury model, SIS, or Cx-SIS film. Cx-CAM significantly suppresses the formation of blood vessels between the peritoneal wall and cecum, as confirmed by CD31 staining. Overall, the newly designed Cx-CAM film works well as an antiadhesion barrier and has better anti-tissue adhesion efficiency.
Highlights
Injury of human tissue induces several physiological responses for self-healing of the injured tissue [1]
The mechanical manipulation and decellularization of cartilage tissue were carried out to remove cartilage-like tissue components and DNA
After treatment with a mixture of HCl and pepsin aqueous solutions, the cartilage acellular-matrix (CAM) powder was solubilized in a suspension form
Summary
Injury of human tissue induces several physiological responses for self-healing of the injured tissue [1]. The physiological responses are a complex process: Infiltration by macrophages, increased permeability of blood vessels, fibrous tissue growth, and tissue repair [2]. This process can induce adhesion between the tissue (at the injury site) and the surrounding healthy tissues or organs through the formation of blood vessels and/or fibrinous adhesions. Postoperative adhesions are quite common and are often a result of injury during and/or after the surgical procedure [3]. Some surgical technologies are intended to minimize the formation of adhesions, postoperative adhesions seem to be an almost unavoidable consequence of surgical intervention
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