Abstract
Abstract Time-dependent assessment for the formation of gold nanoparticles (GNP) within chitosan (CS) matrix and their relative change in size and distribution was examined by monitoring the in-situ reduction of metal ions. UV–Vis spectroscopic analysis confirmed obtainability of GNP with tunable size, density and functionality which conversed from binomial to monomial distribution function of size at an increased concentration of CS. Uniform distribution of ~7 nm nanoparticles was confirmed by TEM image analysis of polymer-metal composites at optimum conditions. XRD analysis of chitosan-gold (CS-GNP) composite coatings confirmed the presence of gold nanoparticles having two (111) and (200) crystal planes. Energy dispersive X-ray spectroscopy and 1H NMR were carried out to confirm the incorporation and uniform distribution of GNP within the composite. The reduction of gold chloride by chitosan was successively achieved in both acidic (cationic environment) and basic pH (anionic environment). A relative change in the % transmittance at peaks attributing to amine group (1579 cm−1) and hydroxyl group (3405 cm−1) was observed in the FTIR spectra of CS-GNP indicating the role of these groups in reduction process at different pH environments. These functionally graded films showed decreased viability and proliferation of human hepatocellular carcinoma cells (HepG2) at in-vitro conditions.
Published Version
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