Preparation, Characterization of Bio-Based Zinc Oxide Nanoparticles and Its Microbicidal Potential over Chemical Based and Commerically Available Drug

Abstract

Background and Objective: Nanotechnology has spread its roots and branches covering every field. Fascinating physcio-chemical properties and functional applications have made zinc oxide nanoparticles (ZnO NPs) one of the most multifaceted materials. Zinc oxide nanoparticles synthesis using concoction of plant is safer and nature cordial due to its non-toxic and clean compared to its chemical counterpart. Present quest involves zinc oxide nanoparticles production by chemical and biological methods (Citrus sinensis peel extract). Materials and Methods: Synthesized zinc oxide nanoparticles were characterized using ultraviolet-visible UV-Visible spectroscopy, Fourier transform infra-red (FTIR) spectroscopy, and scanning electron microscopy (SEM). Synthesized zinc oxide nanoparticles at concentration (0.1g, 0.25g and 0.5g/10ml) were compared against microbial strains Staphylococcus epidermidis and Aspergillus flavus. Results: Synthesized ZnO NP's shows absorption peak at 356 nm in case of biological synthesis and 386 nm in case of chemical synthesized. FTIR proves the presence of alcohol, phenol, nitro compound; alkyne in case of biologically synthesized ZnO NPs and alkene, alkane, alkyl halides in case of chemically synthesized ZnO NPs. SEM confirmed the average size of 25-40 nm. Further, promising results were obtained at 0.5g/10ml of biologically synthesized ZnO NP's in case of Aspergillus flavus and Staphylococcus epidermidis. Conclusion: the biomimetic synthesis of ZnO nanoparticles could be an alternative to chemical synthesis and commercially available drugs.