Abstract
Lymph node cells from rabbits primed with keyhole limpet hemocyanin (KLH) were separated into two populations by passage over goat anti-rabbit Fab′ (AFab′) columns. Cells with readily detected surface immunoglobulin (SIg) were retained on the column whereas those that passed through lacked SIg and receptors for C3. Adherent cells, eluted with purified rabbit IgG or normal rabbit serum (NRS) were mostly SIg+ and enriched for complement receptor lymphocytes (CRL) compared to the starting, or unfractionated population. Adherent LNC incorporated much less [ 3H]thymidine than did non-adherent or unfractionated LNC when stimulated with Con A. When induced with AFab′, however, only adherent and unfractionated LNC incorporated [ 3H]thymidine above background levels. Both non-adherent and adherent LNC from KLH primed rabbits showed a reduced capacity to synthesize anti-KLH antibody, total immunoglobulin, and total protein when induced with KLH in vitro. However, addition of KLH to a mixture of non-adherent and adherent LNC resulted in reconstitution of anti-KLH, total immunoglobulin, and total protein synthesis to the levels induced in unfractionated LNC. It was concluded that the non-adherent LNC consisted almost exclusively of T cells and the adherent LNC of B cells. By a variety of criteria the T cells were suitable for many types of studies of the antibody response. However, the B cells were not always suited for these studies.
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call