Preparation, characterization, and bioevaluation of 99mTc-famotidine as a selective radiotracer for peptic ulcer disorder detection in mice

Abstract

Abstract This work focuses on tracking peptic ulcer localized in mice. The formation of a [99mTc]dithiocarbamate of famotidine complex at optimum conditions of reaction temperature (37 °C), reaction time (30 min), pH of the reaction mixture (5), amount of substrate (100 µg), amount of reducing agent (tin (II) content, 50 µg), was achieved using radioactive Tc-99m (250–400 MBq), with labelling yield of 98% and high radiochemical purity. The in-vitro stability of [99mTc]dithiocarbamate of famotidine complex was shown to be high in rat serum for up to 8 h. Normal and ulcerated mice were used in biodistribution studies. Famotidine works by blocking histamine-2-receptor antagonists (H2RAs). The high absorption of [99mTc]dithiocarbamate of famotidine complex in stomach in amount of 27.15% injected dose/g organ (ID/g) observed in ulcerated mice for up to 24 h demonstrated its usefulness as a novel radiotracer for stomach imaging.