Radioiodinated esomeprazole as a model for peptic ulcer localization

Abstract

Abstract This work focuses on tracking stomach ulcer localized in mice. High labeling yield and radiochemical purity were achieved for the formation of a [125I]esomeprazole radiotracer at optimum conditions of oxidizing agent content (chloramines-T (Ch-T), 100 μg), substrate amount (Esom, 100 μg), pH of reaction mixture (6), reaction time (30 min) and temperature (37 °C), using radioactive iodine-125 (200–450 MBq). The radiolabeled compound, [125I]esom, was stable in serum and saline solution during 24 h. Esom is acting as a histamine-2-receptor antagonist (H2RA). Biodistribution studies were carried in normal and ulcerated mice. High uptake of 78.12 ± 0.80% injected dose/g organ (ID/g) observed in ulcerated mice confirmed the suitability of [125I]esomeprazole as a novel radiotracer for stomach imaging.