Preparation by radiation polymerization of functional microspheres with aldehyde groups

Abstract

Functional microspheres with aldehyde groups were prepared by radiation polymerization of suspended acrolein systems at low temperatures. The particle size of polyacrolein microspheres varied with irradiation temperature and concentration of added monomers such as 2-hydroxyethyl methacrylate. The properties of the microspheres obtained by radiation copolymerization of acrolein and hydrophilic monomers were examined. The ability of the microspheres to bind antibodies was found to depend on the hydrophilicity of the monomers. The microspheres permit covalent binding in one step. The microsphere-antibody conjugate can be used as a cell marker.