Preparation and Properties of Chitosan-Tranexamic Acid Salts

Abstract

The solid chitosan-tranexamic acid salts were successfully prepared by the method of half-wet grinding with chitosan and tranexamic acid as raw materials. The physical properties including water solubility and stability of the prepared samples were tested, and their structures were characterized by Fourier transform infrared (FTIR) spectroscopy and X-ray diffraction (XRD). In addition, the in vitro pro-coagulant activity was evaluated by the test tube method. The results showed that the prepared chitosan-tranexamic acid salts could be dissolved in weak acidic, neutral and weak alkaline aqueous solutions, expanding the dissolution range. The characterization results indicated that the protonation reaction of the chitosan amino group was achieved without solvent and the crystallinity degree of chitosan-tranexamic acid salts decreased by comparison with chitosan. The hemostasis evaluation showed that chitosan-tranexamic acid salts with different mass ratios had pro-coagulant activities in vitro. In particular, the clotting time of chitosan acid salt with mass ratio of 1:2 was shorter than that of Yunnan Baiyao. This effort laid the foundation for the development of the promising chitosan-based hemostatic materials.