Preparation and process optimization of notoginsenoside R1 chitosan nanoparticles

Abstract

Objective To prepare optimization of notoginsenoside R1 chitosan nanoparticles, to provide a theoretical basis for clinical application of the drug. Methods Notoginsenoside R1 chitosan nanoparticles were prepared, HPLC method was used to detect the content of notoginsenoside R1 chitosan nanoparticles, preparation technology of nanoparticles were optimized by orthogonal experiment, and the optimized preparation technology of nanoparticles was verified. Results HPLC standard curve equation was A=911.49C-1803.4(r=0.999 9), linear range was from 25 to 900 g/mL.The intra day precision were 1.520%, 0.884% and 0.969%(n=6), and the inter day precision were 1.591%, 1.447% and 1.269%(n=6). The recovery rates of low, medium and high concentrations were (98.11±1.16)%, (101.27±0.59)% and (100.97±0.82)%.4 factors of orthogonal experiment: the concentration of chitosan, the mass ratio of drug and carrier, temperature and rotational speed, and 3 levels of each factor were selected.The average particle size, encapsulation efficiency and drug loading were selected as control indexes.The test results were determined by the method of comprehensive weighted scoring.The orthogonal design was designed according to L9(34) orthogonal design.The optimization process was 2% of chitosan concentration, 20% of the weight ratio of drug and carrier, 35 ℃ of temperature, 600 r/min of rotational speed.According to the optimized process, the average particle size was (123.40±7.68)nm, the encapsulation efficiency was (58.41±1.59)%, and the drug loading amount was (10.46±0.53)%. Conclusion The optimized preparation process of notoginsenoside R1 chitosan nanoparticles is simple and easy to operate, the entrapment efficiency and drug loading amount were high.As a new dosage form, it has a good clinical application prospect. Key words: Panax notoginseng; Ginsenosides; Chitosan; Nanoparticles; Technology, pharmaceutical