Abstract
Natural polysaccharides are promising candidates for the component of polymer materials for sustainable societies. Here, the preparation of composite films using high- and low-molecular weight (MW) of hyaluronic acid (H-HYA and L-HYA), carboxymethylated HYA (CM-HYA), and high- and low-MW of chitosan (H–CHI and L-CHI) was performed to clarify the effects of the polysaccharide species on formability, mechanical properties, and water-responsive properties of the resulting films. The films prepared using CM-HYA and H–CHI (CM-HYA/H–CHI films) exhibited the highest maximum stress due to more electrostatic interactions between COO − in CM-HYA and NH 3 + in H–CHI. Swelling ratio of CM-HYA/H–CHI films was the lowest both in water and phosphate buffer saline (PBS). Meanwhile, CM-HYA/H–CHI films exhibited the highest moisture absorption ratio and moisture retention capacities, indicating water absorption ability of CM-HYA could be maintained even in the film state. Accordingly, CM-HYA/H–CHI films may be advantageous for applications such as moisture-retaining sheets and biomaterials. • Composite films with hyaluronic acid and chitosan were successfully prepared. • Effects of chemical species on these film properties are discussed. • Carboxymethylated hyaluronic acid (CM-HYA) improved the mechanical properties. • CM-HYA provided the highest moisture absorption ratio and retention capacity. • The composite films may become a candidate for moisturizing sheets.
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