Abstract
Objective To prepare conjugate vaccines by covalently coupling group C meningococcal polysaccharides (CPS) of different molecular sizes and tetanus toxioid (TT),and to determine their immunogenicity in mice.Methods CPS was degraded into different size fragments and conjugated to TT after activated by carbodiimide (EDAC) or cyanogen bromide (CNBr) to prepare conjugate vaccines (CPS-TT).NIH mice were immunized with these conjugate vaccines and both serum anti-CPS and antiTT IgG were determined by ELISA.Results Derivation rates and yields of degraded CPS activated by EDAC were higher than those of degraded CPS activated by CNBr,with 3.9％-5.7％ vs.0.4％-1.2％ and 11.4％-21.3％oo vs.2.4％-11.5％,respectively.CPS-TT prepared with degraded CPS activated by either EDCA or CNBr were all highly immunogenic in mice,but anti-CPS antibody levels in mice immunized with CPS-TT prepared with degraded CPS activated by EDCA were higher than those in mice immunized with CPS-TT prepared with degraded CPS activated by CNBr.Conclusion Degraded CPS activated by EDAC can be used to prepare group C meningococcal polysaccharide conjugate vaccine. Key words: Neisseria meningitidis,serogroup C; Polysaccharides,bacterial; Vaccines,conjugate; Immunogenicity
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