Abstract
Fumonisin B1 (FB1) is one of the mycotoxins produced by Fusarium verticillioides, which was mainly found in corn and related products. FB1 was small molecule with no immunogenicity, so it should be conjugated to carrier proteins such as BSA (bovine serum albumin) or KLH (keyhole limpet hemocyanin) to generate immunogenicity. In this study, conjugate FB1-BSA was used to immunize Balb/c mice, and one hybrid cell line 4G5 excreting monoclonal antibody against FB1 was obtained by fusing mouse Sp2/0 myeloma cells with spleen cells from the immunized mouse. Hybridoma 4G5 was injected into the abdomen of Balb/c mice, and the anti-FB1 mcAb was harvested from ascites and the titer reached 6.4 × 104 after purification with caprylic/ammonium sulfate precipitation (CA-AS) method. The cross-reactivity results showed that anti-FB1 mcAb was highly specific to fumonisin B1, and the affinity was 2.1 × 108 L/M. Indirect competitive ELISA (ic-ELISA) indicated that the linear range to detect FB1 was 1–800 ng/mL with IC50 of 32 ng/mL. The detection limit was 1.0 ng/mL, and the recovery average was 93.75 ± 6.90%. Therefore, the anti-FB1 mcAb excreted by 4G5 can be used to detect fumonisin B1 in corn and related samples.
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