Abstract
A rapid and simultaneous immunochromatographic assay for the detection of zearalenone and fumonisin B1 was developed and optimized. Specific monoclonal antibodies against zearalenone or fumonisin B1, labeled with colloidal gold, were used as probes on an immunochromatographic strip for dual detection. The conjugates of zearalenone and fumonisin B1, as two test lines, were coated onto a nitrocellulose membrane to determine whether the samples contained both mycotoxins. The conditions were optimized, investigating the diameter of the gold nanoparticles, the concentrations of the conjugates, the monoclonal antibodies, and the buffer constituents and materials of the strip. Trehalose and tetronic 1307 were added into the buffer, and 25 nm colloidal gold nanoparticles were considered to give improved performance. The single immunochromatographic strips for either zearalenone or fumonisin B1 did not have cross-reactivity with fumonisin B1, zearalenone, deoxynivalenol or aflatoxin B1. The sensitivity levels were 6 and 50 ng/mL for zearalenone and fumonisin B1 on the dual test strip, respectively. In total, 60 samples (including corn, wheat and feedstuffs) were determined using the dual test strip, indirect competitive enzyme-linked immunosorbent assays (IC-ELISAs) and liquid chromatography–mass spectrometry. The results of strips were shown to have good agreement with the enzyme-linked immunosorbent assays and liquid chromatography–mass spectrometry (LC–MS). The indirect competitive enzyme-linked immunosorbent assays also had a strong correlation with the commercial ELISA kits when compared using spiked samples. The colloidal gold immunochromatographic dual strip could detect samples containing zearalenone and fumonisin B1 rapidly (less than 15 min) and accurately (due to the monoclonal antibody), more easily and at a lower cost compared to other detection methods (chromatography and enzyme-linked immunosorbent assays).
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
