Preparation and characterization of ZnO nanoparticles coated by chitosan-linoleic acid; fungal growth and biofilm assay.

Abstract

This study has been carried out on reviewing the use of new synthetic component of zinc oxide nanoparticles (ZnO NPs) coated by Chitosan-linoleic acid (CS-LA) and to assess Minimum inhibitory concentration (MIC) of nanoparticles on clinical samples and biofilm formation in vitro. At first the synthesized ZnO NPs coated by CS-LA were identified with X-ray powder diffraction (XRD), Scanning electron microscope (SEM), Transmission electron microscope (TEM) and Fourier Transform Infrared Spectroscopy analysis (FTIR).Through in vitro tests, the value of MIC and Minimum fungicide concentration (MFC) of nanoparticles and standard and clinical strains of candida were evaluated in comparison with fluconazole as the control group using the CLSI-M27 method. Finally, biofilm formation was studied using MTT assay. The results showed that MIC50 of fluconazole and nanoparticle in clinical strains was 64 µg/ml and 128 µg/ml, respectively. The MIC of fluconazole and nanoparticle in C. albicans (ATCC10231) was 8 µg/ml and 32 µg/ml respectively. The MFC of nanoparticle and fluconazole for clinical samples was recorded at similar level (128 μg/ml). MTT results indicated that the capacity of inhibition of biofilm formation was 43.07 % and 36.68 % by ZnO NPs and fluconazole, respectively. It is concluded that the new synthesized nanoparticle has appropriate efficacy compared with fluconazole in inhibitory activity on C. albicans growth and biofilm formation. As a result, ZnO NPs can be introduced as an effective agent for diminishing adhesion capacity of C. albicans (Tab. 1, Fig. 4, Ref. 26).