Preparation and characterization of pH-sensitive chitosan/starch/MoS2 nanocomposite for control release of curcumin macromolecules drug delivery; application in the breast cancer treatment

Abstract

In this work, chitosan (CS), Starch (S), and Molybdenum Disulfide (MoS2) were combined to create a nanocarrier that was utilized to treat breast cancer using the MCF-7 cell line. To analyze the features of the nanocarrier, Fourier-transform infrared spectroscopy (FTIR) and X-Ray diffraction (XRD) tests were performed, respectively, to discover physical interactions and chemical bonding. Field emission scanning electron microscopy (FE-SEM), Dynamic light scattering (DLS), and zeta potential analyses were performed and reported to determine the structural characteristics and morphology of nanoparticles, size distribution, and surface charge of nanocarriers, respectively. The average size of the nanocomposite was measured at around 279 nm, and the surface charge of the nanocarrier was determined to be +86.31 mV. The entrapment and drug loading efficiency of nanocarriers were 87.25 % and 46.5 %, respectively, which is an acceptable value. The kinetics and release mode of the drug were investigated, and it was found that the synthesized nanocarrier was sensitive to pH and that its release was stable. The amount of the nanocarriers' toxicity and cell death were evaluated using MTT tests and flow cytometry, respectively. In the present study, the nanocarrier was wholly nontoxic and had anticancer properties against the MCF-7 cell line. This nanocarrier is very important due to its non-toxicity and sensitivity to pH and can be used in drug delivery and medical applications.