Chitosan-Carbon nanotube Composite: An approach for controlled release of Quercetin, Modified with carboxymethyl Cellulose, for potential Anti-Cancer therapy

Abstract

Nanoparticles as medicine has made progress in the field of medicine and pharmacy by improving the performance of the drug and reducing its consumption. Different materials such as natural and synthetic polymers, metal particles, lipids, etc. are used to produce nanoparticles, which can have different shapes (morphologies) and sizes according to their production and synthesis method. According to the things mentioned in the present study, a new nanocarrier based on hydrogel nanocomposite containing chitosan (CS), carboxymethyl cellulose (CMC) and carbon nanotubes (CNTs) was used to load the drug quercetin (QC), which has antioxidant, antiviral, and anticancer properties. Based on the experiments conducted in the current research and Field emission scanning electron microscopy (FESEM) images and zeta potential analysis, all of them investigate the nanocomposite surface and nanocarrier stability, and the existence of nanocomposite materials is also confirmed by X-ray diffraction analysis (XRD) and Fourier-transform infrared spectroscopy(FTIR) analyses. In the current study, the First-order model analyzes and characterizes the release data far better than the zero and Second-order models do, as shown by the values of R2. Also, the introduction of CNTs improved the drug loading by 48.0% and increased its encapsulation by 86.5%. Also, in the current study, the cytotoxicity of nanocarriers with QC was measured by MTT analysis, and by examining the results, it was found that the significant cytotoxicity of CS/CMC/CNTs/QC on MCF-7 cell line is caused by it compared to free QC. The inhibitory effect of CNT nanoparticles and improving the release behaviour of nanocarriers. Increasing the sustained releaseof QC from CS/CMC/CNTs/QC improves cell apoptosis and decreases viability, and also the late apoptotic cells ratio in CS/CMC/CNTs/QC in the cell apoptosis assay is superior to the cell size samples, which may be due to the controlled and slow release of QC from CS/CMC/CNTs/QC.