Preparation and characterization of collagen coated cholesterol-free liposome nanoparticles matrix for drug carriers and tissue engineering application

Abstract

The collagen coated cholesterol (CH)-free liposome nanoparticle (CCLNP) matrix has been prepared and characterized for use as therapeutic drug carriers, tissue engineering and regenerative medicinal applications. The CH-free liposome nanoparticle (LNP) was prepared by heating method under the N2 atmosphere and CCLNP matrix was prepared by incubation of bovine Achilles tendon collagen with preformed CH-free LNP. The results indicate that collagen stabilizes the original liposomal structure, and CCLNP are more stable in vitro than control liposome. This mineralized collagen composite matrix could be useful to bone and dental implants.