Abstract
Abstract Ca2+-free Methanol dehydrogenase (MDH) has been prepared from Hyphomicrobium denitrificans A3151 and spectroscopically characterized. Ca2+-free MDH had full-oxidized pyrroloquinoline quinone (PQQ). Incubation of Ca2+-free MDH with Ca2+ ion led to reconstituted MDH containing full-reduced PQQ, which was tightly bound with Ca2+ ion and had the enzyme activity.
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