Preparation and bioactive properties of bone-repair composite scaffolds based on bioactive glass/hydroxylapatite/chitason

Abstract

The aim of this work was to develop a bioactive glass/hydroxylapatite/chitason composite material for bone repair. Different proportions of bioactive glass/hydroxylapatite/chitason mixture was make into scaffolds by the freeze-drying method. The microstructure and composition were observed and analysed. The third-generation rabbit mesenchymal stem cells were inoculated into the stent. The adhesion of the stent was detected, and the proliferation of cells on the stent was detected. The compatibility between the stent and cells was evaluated. The size and shapes of the prepared bioactive glass/hydroxylapatite/chitason stent were consistent with moulds. The stent has parous structure and no aggregation of bioactive glass was found. The rabbit mesenchymal stem adhered to the stent, and some cells extended and stretched out pseudopodia. Obvious proliferation of bone marrow stromal cells was found on the stent. The prepared bioactive glass/hydroxylapatite/chitason stents have good porosity, high mechanical strength, and good cell compatibility.