Abstract
Since 1975, the technique of cell hybridisation or fusion has been applied to a large number of problems in biomedical science. In recent years the monoclonal antibody has been used in forensic science. Ryouichi Tsuda (1984) [1] identified seminal stain by sandwich ELISA using monoclonal gamma-sm antibody. Sensabaugh (1978) [2] successfully found a human semen specific protein (HSSP) from human semnial plasma. The HSSP was proved to be a 30000-dalton semen-specific glycoprotein of prostatic origin and thus named as P30. The present article describes the method of preparation of monoclonal P30 antibody and its application to the examination of semen or seminal stain.
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