Prenatal, lactation and postnatal effects of lead acetate on histological, histochemical and hepatic indices of liver of male offspring wistar rats

Abstract

The aim of this study was to investigate the possible effects of maternal administration to lead acetate on the morphological structure of the liver; AST, ALT, ALP and GGT levels and glycogen content of hepatocytes of male offspring wistar rats. Female rats were exposed to 0.2% lead acetate in drinking water and the study was performed on their male offspring (7 groups). To evaluate the levels of liver enzymes, blood samples were taken from rats after anesthesia. After dissection, to study its morphological structure and glycogen content of hepatocytes, liver tissue was isolated and transferred to 10% formalin. To measure the levels of hepatic enzymes; AST, ALT, ALP and GGT; serum samples were evaluated. For histological examinations, liver tissue samples were embedded in paraffin after tissue processing and 3 μm thickness sections were prepared from them and then H&E and PAS staining was performed. The results showed that exposure to 0.2% lead acetate induced significant changes in liver enzyme levels in different experimental groups.