Prenatal exposure to bisphenol S and altered newborn mitochondrial DNA copy number in a baby cohort study: Sex-specific associations

Abstract

Bisphenol S (BPS) is a main substitute for bisphenol A, which are ubiquitous in human daily products. Newborn mitochondrial DNA copy number (mtDNAcn) is considered as a marker for biological aging and human health, and has been related to diseases in later life. We recruited 762 mother-newborn pairs in a birth cohort study between 2013 and 2015 in Wuhan, China. Urinary BPS concentrations were detected using ultra-high-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). MtDNAcn from cord blood was measured by quantitative real-time polymerase chain reaction (qPCR). We applied multiple informant models based on generalized estimating equations to assess the associations between prenatal BPS exposure and mtDNAcn. The median urine concentrations of BPS were 0.32 μg/L, 0.34 μg/L, and 0.36 μg/L in the first, second, and third trimesters, respectively. In the multiple informant models, we observed significant associations between BPS and mtDNAcn among male newborns. Compared with the lowest quarters, the second, third, and the highest quarter of BPS level were associated with 58.00% (95% CI: 76.58%, −24.66%), 64.65% (95% CI: 79.40%, −39.33%) and 59.07% (95% CI: 75.16%, −32.58%) reductions of mtDNAcn in the first trimester, respectively. No significant associations were found in the second and third trimesters. The associations between BPS and mtDNAcn were not found among female newborns. Findings from this study suggested that BPS exposure was related to decreased mtDNAcn in male newborns. The first trimester was identified as the critical windows for BPS exposure during pregnancy.