Abstract
The rambutan peel (RP) is a relevant source of bioactive molecules, which could be used for application in cosmetics, food, and pharmaceutical areas. Total soluble polyphenol content was extracted from Mexican variety rambutan peels using an emergent ultrasound/microwave-assisted extraction (U/M-AE) technology. Five extractions were performed using different mass/volume and ethanol/water ratios; 1:16-0; 1:16-70; 1:8-0; 1:8-70; 1:12-30. Condition 1:16-0 was defined as the best extraction condition with 0% ethanol percentage (only water). The content of total soluble polyphenols was 307.57 mg/g. The total bound polyphenol content was 26.53 mg/g. Besides, two separation processes were made with the soluble fraction; the first one was performed using Amberlite XAD-16 (Sigma-Aldrich, Saint Louis, MO, USA), and seven polyphenolic compounds were obtained. The second one was performed using a preparative HPLC (Varian, Palo Alto, CA, USA) equipment obtained fraction where three compounds were obtained: geraniin (main compound), ellagic acid, and ellagic acid pentoside. The major compound isolated in the two separations was geraniin, according to HPLC/ESI/MS (High Performance Liquid Chromatography/ElectroSpray Ionization/Mass) analysis.
Highlights
The rambutan (Nephelium lappaceum L.) is an exotic fruit that is grown in Southeast Asia (Malaysia, Thailand, Indonesia)
HHDP(Hexahydroxydiphenic (Hexahydroxydiphenic acid) group, particular group of ellagitannins; geraniin, an ellagitannin; (c) ellagic acid, a compound derived from ellagitannins
Geraniin, an ellagitannin; (c) ellagic acid, a compound derived from ellagitannins
Summary
The rambutan (Nephelium lappaceum L.) is an exotic fruit that is grown in Southeast Asia (Malaysia, Thailand, Indonesia). Chromatography/Mass) analysis, Chromatography/Mass) analysis, has has advantages advantages for the identification of bioactive molecules because extraction technology allows a better between between the solvent andsolvent the compounds the use useofofthis this extraction technology allows a interaction better interaction the and the of interest due the cavitation phenomenon produced by ultrasound. This interactionThis is favored compounds of to interest due to the cavitation phenomenon produced by ultrasound. Bioactive (geraniin), liquid chromatography and mass spectrometry (HPLC/ESI/MS)
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