Preliminary study to investigate cellulose biodegradability of Bacillus - Aspergillus and Neurospora Crassa on cassava peels (Manihot Esculenta)

Abstract

Cassava peel holds an abundance of nutrients that could be utilized as fertilizer in agriculture. However, high cellulose contents make it hard for organic digestion, thus slow down the absorption for plants. In this study, we investigate microorganisms for effective cellulose degradation of cassava peel waste collected directly from Hung Duy Tapioca Starch Factory in Tay Ninh Province, Viet Nam. Experiments evaluate the decrease of mass cassava peel of the two groups of Bacillus - Aspergillus and positive control strains such as Neurospora Crassa. The sample was tested on Czapek - Dox liquid culture medium, a method of assessing the dry mass difference of samples used to assess biodegradability in 28 days. Besides, tests to assess the production of products and environmental parameters such as glucose and pH were recorded. Initial results showed that the combination of Bacillus and Aspergillus provides the highest glucose concentration, reached 16.52 mg/ml and digests 53% of the cassava mass compared with a combination of Neurospora Crassa reached 11.45 mg/ml and 46% of the cassava mass. Comparison results also showed that the action potential of microorganisms was stronger when combined and provided evidence that the formation of combinations of microorganisms would work well in probiotics, which brings better environmental treatment efficiency.