Abstract
Objective To investigate whether allo-or xenotransplantation tolerance can be induced by transplanted thymus combined with metanephros primordia, from rats to cyclophosphamide (CP) pretreated recipients. Methods Two pairs of thymus and metanephros primordia were extracted from the Lewis rats on embryonic day16 (E16) and implanted into experiment (Ⅰ) group or control (Ⅱ, Ⅲ, Ⅳ) group renal capsules and omenta of thymectomized recipients, which had been pretreated with CP (80 mg/kg in BN rats and 200 mg/kg in C57BL/6 mice, ip, q6d×4). Post-transplantation, subcutaneous injection (sc, qd×12 d) of cyclosporine [8 mg/(kg·d) in BN rat and 12 mg/(kg·d) in C57BL/6 mouse] were simultaneously administrated till the 13th day. Histopathological examination of thymus and metanephros grafts and functional of developed metanephroi were performed post-transplantation. Rat seruminterleukin (IL)-2 and interferon-γ (IFN-γ) levels, one-way mixed lymphocyte reaction (MLR) and skin transplantation tests were measured. Results On the 14th day post-xenotransplantation, the thymus and metanephros primordia of Lewis rats were not found in the C57BL/6 mouse. On the 21st day after allotransplantation, the thymus and metanephros of some experimental (Ⅰ) group developed well, displaying partial urinary function. In experimental (Ⅰ) group, Banff rejection score (χ2=6.316, P 0.05) and SI had no significant difference (F=0.920, P>0.05). 5-7 weeks post-transplantation, acute rejection of metanephros and thymus deteriorated and connective tissue scars gradually formed. Conclusion Using the CP pretreatment protocol, E16 Lewis thymus and metanephros anlagens xenografted into C57BL/6 mice failed to grow and function, whereas fail to induce allogeneic immune tolerance but can grow and develop in the peritoneal cavity of BN rat, produce donor-specific immune hyporesponsiveness. Key words: Embryo; Thymus Primordia; Metanephros primordia; Transplantation; Tolerance
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