Preliminary study for investigation of G. anatis in broilers Abdelazeem M. Algammal1, Wafaa A. Abd El-Ghany2, Amira A. Elewa3, and Ahmed M. Hamouda3

Abstract

Gallibacterium anatis is recognized as a globally distributed species of bacteria that has been isolated from birds . The aim of the present prelaminary study is to investigate the prevalence of G. anatis isolated from diseased chickens from private commercial broiler farms at Ismailia governorate, Egypt. A total of 80 tracheal swabs obtained from broiler chickens suffering from respiratory manifestations. The collected specimens were subjected to bacteriological examination and the prevalence of G.anatis was 35%. All obtained isolates were positive for oxidase, catalase, nitrate reduction, sucrose, sorbitol, and mannitol fermentation tests. However, the obtained isolates were negative for citrate utilization, indole, urease, gelatinase and maltose fermentation tests. Besides, the recovered isolates were negative for the motility test. The gold standard method of bacterial isolation and identification( including culture characters, hemolytic activity on blood agar, morphological characters, motility test, and biochemical characteristics) is essential for characterization of G. anatis in birds.