Abstract
New media for the demonstration of gas and indole production in a single tube were compared with media recommended by Report 71 for use in the 44°C confirmatory test for E. coli. The results demonstrate that the use of mannitol can reduce the numbers of false positive and false negative gas reactions. Indole-production tests may be performed in the same tube as the mannitol fermentation test by including tryptone in the medium. Tryptone mannitol ricinoleate broth and tryptone mannitol broth were found to be most suitable for the confirmation of E. coli in coliform presumptive tubes and membrane filter colonies respectively. The results of the mannitol fermentation and indole-production tests are available within 24 h at 44°C. The media are inexpensive and simple to prepare.
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