Abstract
The purpose of this study was to investigate the antidiabetic effect of phytocompounds from Swietenia macrophylla seed using preliminary phytochemical screening, invitro antioxidant activity and molecular docking studies. The powdered seed extract of Swietenia macrophylla was to investigate the phytochemical screening exhibited the presence of alkaloid, phenols, tannins, flavonoids, terpenoids, steroids, carbohydrates, amino acids and proteins as major active constituents. The antioxidant activity of Swietenia macrophylla seed was evaluated by DPPH free radical scavenging assay. Rutin was used as a reference compound. The Swietenia macrophylla seed exhibited 56.0471% of free radical scavenging activity as compared with rutin. The molecular docking studies performed by using molecular docking server online respectively in which the antidiabetic target namely glutamine:fructose-6-phosphate amidotransferase (GFAT) (PDB id: 2ZJ3) have a potential interaction with swietenine, swietenolide, β-sitosterol, and fucosterol. In this study, the protein glutamine:fructose-6-phosphate amidotransferase (GFAT) was used from its structure perspectives. Its primary and secondary structures were evaluated using online tools. Its role in antidiabetic was assessed by molecular docking the compounds present in the seed extract of Swietenia macrophylla assayed by GC-MS analysis. This in-silico study demonstrates the interactions of active components of Swietenia macrophylla against Type I and Type II diabetes.
Highlights
Diabetes mellitus (DM) is a set of diseases that results high levels of blood glucose and depends on insulin production
The protein sequence was subjected for comparative homology modeling via Swiss model [26] and evaluate by Rampage online server [27]
The performance of the powdered seed extract of Swietenia macrophylla once reacted to chemical reagents demonstrates the characters individuality for observing the phytoconstituents
Summary
Diabetes mellitus (DM) is a set of diseases that results high levels of blood glucose and depends on insulin production. It involves various disorders of lipids and protein metabolism [1]. The use of complementary and alternative medicine and especially the consumption of botanicals have been increasing rapid worldwide, mostly because of the supposedly less frequent side-effects when compared to modern medicine [8] One such plant is Swietenia macrophylla is a folkmedicinal tree known as Mahogany. An attempt has been taken to investigate the antidiabetic activity of identified bioactive compounds of Swietenia macrophylla seed on type II model through molecular docking. The widespread uses of Swietenia macrophylla in traditional medicine have resulted in significant qualitative analysis of the plant and its active principles
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