Preliminary Phytochemical Screening and Antimicrobial Studies on the Aerial Parts Extracts of Crinum latifolium L. (Amaryllidaceae)

Abstract

The present study was undertaken to find the antimicrobial activities, phytochemical presence in various aerial parts extract of Crinum latifolium (C. latifolium). Qualitative analysis of various phytochemical constituents and quantitative analysis of total phenolics, flavonoids and alkaloids was determined by the well-known test protocol available in the literature. Quantitative analysis of phenolic, flavonoids and alkaloids was carried out by Folins Ciocalteau reagent, aluminium chloride and bromocresol green method respectively. The in vitro antimicrobial activity was done by well diffusion assay method against S. mutans and S. bongori using standard ofloxacin, ciprofloxacin (10-30μg/ml). The antimicrobial activity was determined by measuring the diameter of the zone of inhibition in term of millimeter (mm). The phytochemical analysis showed the presence of tannins, glycosides, flavonoids and alkaloids ect. The antimicrobial activity of hydroalcoholic extract of aerial parts against all microorganisms was concentration dependent manner but less than standard drug. It is concluded that the antimicrobial activity showed by the plant is due to the presence of these phytochemicals. For future studies, phytochemcials responsible for these activities can be isolated and modified for pharmacological purpose.