Preliminary in-vitro investigation of magnetic fluid hyperthermia in cervical cancer cells

Abstract

The present study reports auto-tunable temperature sensitive iron-based magnetic nanoparticles fluid synthesized for its application in magnetic fluid hyperthermia. The effect of magnetic field and concentration of the sample is investigated for the induction heating at 350 kHz frequency. The concentration of magnetic fluid and magnetic field was optimized based on the specific absorption of the sample. The cytotoxicity of the optimized magnetic fluid was analyzed on cervical cancer cell line HeLa with and without magnetic field applicator by performing MTT assay. The cytotoxicity assay after induction heating for the magnetic fluid showed cell death up to 75% for 24 h of interaction of fluid with cells. Upon increasing the time of interaction of magnetic fluid to 36 h and for 60 min hyperthermia heating session, almost all cells were found dead. The preliminary analysis revealed significant efficacy of the synthesized magnetic fluid to caused cell death due to hyperthermia of one hour session. In future such type of magnetic fluids can be used for localized treatment of cancer under magnetic field.