Abstract
Summary In two experiments with artificial inoculation, the efficacy of imazalil (Fungazil 100 SL) and biopreparation Serenade ® (Bacillus subtilis QST 713) for protection of mushroom culture against green mould (Trichoderma aggressivum f. europaeum) and cobweb disease (Cladobotryum dendroides) was evaluated. Biopreparation Serenade used at different rates for treatment of mushroom grain spawn or introduced into phase II mushroom compost, was totally ineffective in controlling the green mould disease. This product, applied at the dosages of 1, 2 and 4 g/m 2 as a drench on the surface of the casing soil, also failed to control the cobweb disease. Also imazalil, used for treatment of mushroom spawn, was not effective against the green mould disease. Moreover, this application technique of imazalil had strongly inhibited the growth of mushroom mycelium. In contrast, the application of imazalil at the rates of 0.6 and 1.2 g/m 2 on the surface of the casing provided a good efficacy in controlling C. dendroides, 71 and 93%, respectively, and did not affect the development of mushroom mycelium. Following the application of this fungicide by drench method, imazalil residues in sporocarps were below the maximum residue level (MRL).
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