Abstract
Objective The study was to determine plasma myeloperoxidase (MPO), oxidized low density lipoprotein (OX-LDL) and lipoprotein-associated phospholipase A2 (LP-PLA2) levels in Beijing healthy adults and investigate the correlation among these three biomarkers and risk factors of cardiovascular disease. Methods One thousand one hundred and fifty-five healthy subjects, including 567 male and 588 female, were selected from Center of Health Examination of Armed Police General Hospital during the period of February to October 2015, who range in age from 18 to 87. Plasma MPO, OX-LDL and LP-PLA2 levels were determined by enzyme linked immunosorbent assay (ELISA). Groups were set by gender and age according to C28-A2 standard of Clinical and Laboratory Standards Institute (CLSI) and series of guiding principles of performance evaluation in vitro diagnostic reagent. Male and female subjects were classified into four subgroups by age respectively as those who were from 18 to 29,≥30 to 39,≥40 to 49,≥50. The correlation among levels of MPO, OX-LDL and LP-PLA2 was achieved by regression analysis method. Results One thousand one hundred and fifty-five healthy subjects of MPO reference value was 94.1 μg/L, while OX-LDL and LP-PLA2 reference range were 14.08-127.58 mg/L and 47.30-218.96 μg/L respectively. Experimental results showed that these three biomarkers in plasma of healthy subjects increased with the growing age. Male and female did not need to set up independent subgroups of reference range, but there was significant difference in plasma OX-LDL and LP-PLA2 between male and female (t value were 2.134, 2.381; P 0.05); Meanwhile, there was significant correlation among MPO, OX-LDL and LP-PLA2 (r value were 0.181, 0.174, 0.470; P<0.01). Conclusion Reference ranges of plasma MPO, LP-PLA2 and Ox-LDL have been set up for Beijing healthy adults, which can be used clinically as biomarkers of cardiovascular disease. Key words: Myeloperoxidase; Oxidized low density lipoprotein; Lipoprotein-associated phospholipase A2
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