Abstract
The chemical constituents of the roots of Smilax bockii Warb. were investigated and two pregnane glycosides and three steroid saponins were isolated. Their structures were established as 3β-hydroxypregna-5,16-dien-20-one-3-O-α-L-rhamnopyranosyl(1 → 4)-α-L-rhamnopyranosyl(1 → 4)-[α-L-rhamnopyranosyl(1 → 2)]-β-D-glucopyranoside (1), 3β-hydroxypregna-5,16-dien-20-one-3-O-α-L-rhamnopyranosyl(1 → 2)-[α-L-rhamnopyranosyl(1 → 4)]-β-D-glucopyranoside (2), dioscin (3), methyl protodioscin (4), 26-O-β-D-glucopyranosyl-22α-methoxyl-(25R)-furost-5-en-3β, 26-diol 3-O-α-L-rhamnopyranosyl(1 → 4)-α-L-rhamnopyranosyl(1 → 4)-[α-L-rhamnopyranosyl(1 → 2)]-β-D-glucopyranoside (5) on the basis of spectral analysis and chemical methods. Compound 1 is a new natural product and compounds 2, 5 were first isolated from the genus Smilax. Compound 5 showed enhancing activity of nerve growth factor (NGF)-induced neurite outgrowth in PC 12D cells.
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