Abstract
Ghost peaks in liquid chromatography (LC) with mixed eluents can be brought about by preferential solvation of solute by one of the components of eluent. The preferential solvation causes the change of composition of “dialyzed solvent,” i.e., of the bulk solvent outside solvate shells of solute molecules. In the chromatographic column, the zone of dialyzed solvent is separated from the solute molecules and corresponding ghost (vacant) peak occurs. The vacant peaks of preferential solvation: (a) complicate the interpretation of chromatograms; and (b) may be used in the quantitative evaluation of the phenomenon of preferential solvation. The occurence of vacant peaks of preferential solvation is demonstrated in the system Sephadex LH-20 — benzene/methanol 77.8/22.2 v/v — by injecting a series of polymers, oligomers, and low molecular substances.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
