Abstract
The sizes of mRNAs of various defense proteins of Sarcophaga peregrina (flesh fly) were shown to become progressively shorter. We studied the shortening of the Sarcophaga lectin mRNA, because this mRNA shows acute phase expression on injury of the larvae and programmed expression in the pupal stage. Results showed that this mRNA underwent deadenylation when expressed in response to body injury, but no appreciable change in size during programmed expression in the pupal stage. During acute phase expression, the size of the poly(A) tail of the Sarcophaga lectin mRNA decreased from 130 bases to about 30 bases in 6 h. The half-lives of the forms with different sizes of poly(A) tail were almost identical.
Published Version
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