Preexisting frequency of antigen-specific naive T cell dictates immunodominance of antigen-specific CD8+ T cells (43.25)

Abstract

Abstract Immunodominance is a common feature of Ag-specific CTL responses to infection or vaccines. Understanding the basis of immunodominance is crucial to understanding cellular immunity and viral evasion mechanisms and will provide a rational approach for improving vaccine design. This study was performed comparing CTLs specific for the SIV Gag p11C (dominant) and SIV Pol p68A (subdominant) epitopes that are consistently generated in Mamu-A*01+ rhesus monkeys exposed to SIV proteins. Additionally, vaccinated monkeys were used to prevent any issues of antigenic variation or dynamic changes in CTL responses by continuous Ag exposure. Analysis of the TCR repertoire revealed the usage of higher numbers of TCR clones by the dominant p11C-specific CTL population. Preferential usage of specific TCRs and the in vitro functional TCR-α and -β chain-pairing assay suggests that every peptide/MHC complex may only be recognized by a limited number of unique combinations of α and β chain pairs. The wider array of TCR clones used by the dominant p11C-specific CTL population might be explained by the higher probability of generating those specific TCR chain pairs. Our data suggest that Ag-specific naïve T cell precursor frequency may be predetermined and that this dictates immunodominance of CD8+ T cell responses. These findings will aid in understanding immunodominance and designing new approaches to modulate CTL responses.