Prediction of structure and functions from full length coding sequence ofSRYgene in crossbred and Indicine bulls

Abstract

Sex-determining Region on Y chromosome (SRY) is testis determining factor in mammalian species. It encodes a protein that possess high mobility group (HMG) DNA-binding domain and acts as an architectural transcription factor. Mutation in this gene causes sex-reversal in many mammalian species. Detailed structure and exact biological functions of this protein in bovine species are not known. In the present study the full length coding sequences of the gene from crosssbred and indicine bulls were amplified, translated in silico and 3D model of the protein were predicted from the amino acid sequences. Predicted structure-activity relationship of the protein states that the protein is subcellular, essential, N-glycosylated in nature and is involved in sex determination and neuroendocrine functioning in central and peripheral nervous systems. The proteins have also plausible role in RNA metabolism, transition-metal and calmodulin binding. Overall this structural and functional information about the protein in bovine species will reinforce the understanding of domain-specific role of this protein and development of diagnostics for sex-determination in bovine species.