Abstract
A model has been developed for predicting the effect of transition metals as components of the mobile phase in high-performance liquid chromatography on the retention of an analyte containing a chelating group. It takes into account the secondary chemical equilibria in solution, including the formation constants of the complexes, the pH of the mobile phase and the concentration of the metal ions. The model was tested by comparison with data determined for the retention of 2-aminophenol on a porous polystyrene-divinylbenzene polymer column in the presence of metal ions.
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