Abstract
BackgroundIleus and postoperative ileus (POI) are common complications of colorectal cancer (CRC). However, little is known about the gut microbiota associated with ileus.MethodDifferences in gut microbiota were evaluated by 16S rRNA gene sequencing. We characterized the gut microbiota in 85 CRC patients (cohort 1) and detected differences, and an independent cohort composed of 38 CRC patients (cohort 2) was used to evaluate the results.ResultsThe gut microbiota of CRC patients with and without ileus exhibited large differences in alpha- and beta-diversities and bacterial taxa. The Firmicutes-to-Bacteroidetes ratio and microbial dysbiosis index (MDI) showed greater dysbiosis among ileus patients than among those without ileus. According to the location of CRC, the difference in gut microbiota between patients with and without ileus was more obvious in those with distal CRC than in those with proximal CRC. Finally, Faecalibacterium was significantly reduced in the postoperative perioperative period in patients with ileus. Thus, we used Faecalibacterium as a biomarker for predicting perioperative or POI: the AUC value was 0.74 for perioperative ileus and 0.67 for POI that appeared at 6 months after hospital discharge. The predictive power was evaluated in Cohort 2, with an AUC value of 0.79.ConclusionThese findings regarding difference of gut microbiota in postoperative CRC patients may provide a theoretical basis for the use of microbiota as biomarkers for the prediction of POI.
Highlights
Colorectal cancer (CRC) has the third highest incidence and second highest mortality rate among cancers [1]
The postoperative patients were further divided into subgroups of 10 patients with perioperative ileus and 9 with postoperative ileus (POI)
Operational taxonomic units (OTUs)-level richness between the two groups, even though the results indicated that overgrowth of certain bacteria altered the evenness of the gut microbiota in ileus patients
Summary
Colorectal cancer (CRC) has the third highest incidence and second highest mortality rate among cancers [1]. CRC recurrence after surgical resection is the main cause of treatment failure, and overall recurrence rates are significantly higher after obstruction and subsequent emergency resection than after elective resection [3]. Emerging evidence suggests that gut microbial dysbiosis is an important environmental factor contributing to CRC [4,5,6]. With the development of next-generation sequencing technology, recent studies have found a significantly negative correlation between microbiota richness and colon transit rate [10]. Ileus and postoperative ileus (POI) are common complications of colorectal cancer (CRC). Little is known about the gut microbiota associated with ileus
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
