Abstract

The innovation of next-generation sequencing (NGS) techniques has significantly reduced the price of genome sequencing, lowering barriers to future medical research; it is now feasible to apply genome sequencing to studies where it would have previously been costinefficient. Identifying damaging or pathogenic mutations in vast amounts of complex, highdimensional genome sequencing data may be of particular interest for researchers. Thus, this paper’s aims were to train machine learning models on the attributes of a genetic mutation to predict LoFtool scores (which measure a gene’s intolerance to loss-of-function mutations). These attributes included, but were not limited to, the position of a mutation on a chromosome, changes in amino acids, and changes in codons caused by the mutation. Models were built using the univariate feature selection technique f-regression combined with K-nearest neighbors (KNN), Support Vector Machine (SVM), Random Sample Consensus (RANSAC), Decision Trees, Random Forest, and Extreme Gradient Boosting (XGBoost). These models were evaluated using five-fold cross-validated averages of r-squared, mean squared error, root mean squared error, mean absolute error, and explained variance. The findings of this study include the training of multiple models with testing set r-squared values of 0.97.

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