Preclinical research of the experimental preparation “Ferosel T”

B.V Gutyj,D.F Gufriy,I.I Hariv,R.I Khomyk,R.O Vasiv,V.B Todoriuk,V.M Hunchak

doi:10.32718/ujvas1-1.01

Abstract

The results of preclinical studies of the new ferrodextran preparation “Ferosel T”, which contains ferrum and selenium, are presented. It has been established that at oral administration of ferrocellus T in a prophylactic dose of 2 ml/kg b.w. and the therapeutic dose of 4 ml/kg for 3 days in a row in the stomach of laboratory rats did not show toxic effects. No toxic effect of T ferrocellus is established at introducing it into the stomach in doses in 3 and 10 times higher than the therapeutic ones. Under conditions of subcutaneous administration of the drug, the death of white rats was not observed, only short-term inhibition of laboratory animals, which was prescribed the drug in a dose of 10 ml/kg b.w. It was established that in the preventive and optimal therapeutic doses the drug did not affect the detoxification function of the liver. In rats, which ferrocellus T was administered at maximum therapeutic and possibly toxic doses, the duration of hexenal sleep was for 32 and 35% higher relative to control values. The investigation of the emotional and behavioral reactions of laboratory animals after administration of ferrocellus T for 21 days in therapeutic and maximum therapeutic doses did not show a significant effect on the nervous system. In terms of hyperemia and swelling of the skin and the thickness of the skin, ferrocellus T in prophylactic and therapeutic doses upon application to rabbit skin did not cause local irritation. Separate injection of the drug “Ferosel T” by sub-planar way to guinea pigs in 0.1 ml. did not cause swollen reactions of the paws. As a result of the conducted research, no allergenic properties of the drug “Ferosel T” were found. In laboratory rats, which were introduced ferrosel T in the prophylactic dose of probable changes in the weight of the heart, liver, spleen and kidneys is not established. In rats, which were administered ferrocellus T in an optimal therapeutic dose, in comparison with control weights of the spleen and liver, respectively, was in 10.3 and 6.4% higher. When introducing ferrocellus T at the maximum therapeutic dose, the mass of the spleen and liver was in 14.0 and 15.0% higher, respectively. The results of the studies indicate that the drug “Ferosel T” is safe when used for the prevention and treatment of animals.

Highlights

In order to conduct the treatment and prevention of ferrum deficiency anemia of piglets, we have proposed a new ferro dextran drug “Ferosel T” – an analogue of the officinal preparation “Ferrovet 7.5%”, from which the ferrocellus-T is different in the presence of sodium selenite in it (Todoriuk et al, 2016)
For the treatment and prevention of ferrum deficiency anemia of piglets, we proposed a new saline ferro dextran drug “Ferosel T” – an analogue of the officinal preparation “Ferrovet 7.5%”, from which the ferrocellus T is different by the presence of sodium selenite (Todoriuk et al, 2016)
Taking into account the fact that the toxicity of the components of the drug Ferosel T, in particular Ferrum and Selenium, is already known and published in the literature (Gutyj et al, 2016; Khariv et al, 2016; Martyshuk et al, 2016; Khariv et al, 2017; Sobolev et al, 2018), we investigated the acute and chronic toxicity of Ferosel T as a whole, for oral and parenteral administration, and studied its effect on the detoxification function of the liver

Summary

Introduction

Ukraine as of December 16, 1996, each new preparation recommended for the treatment of animals must meet the According to reports in the literature, with the intensive following requirements: introduction of pigs on an industrial basis, in the absence of the new drug, in comparison with the analogue prodtimely prophylactic measures, the disease of newborn pig- uct, should exhibit higher therapeutic efficacy; lets to ferrum-deficient anemia is 100%, and death reaches 35% (Zimmermann, 1995; Lee and Downing, 1979; Rytych et al, 2012; Ohorodnyk, 2013; Berezovskyi et al, 2013; Starzyński et al, 2013; Danchuk et al, 2013; Pu et al, 2015; Hunchak et al, 2018). 2017; 2018), pharmacological action and pharmacotherapeutic efficacy of ferrocellus T at spontaneous ferrum deficiency anemia of piglets. Taking into account the fact that the toxicity of the components of the drug Ferosel T, in particular Ferrum and Selenium, is already known and published in the literature (Gutyj et al, 2016; Khariv et al, 2016; Martyshuk et al, 2016; Khariv et al, 2017; Sobolev et al, 2018), we investigated the acute and chronic toxicity of Ferosel T as a whole, for oral and parenteral administration, and studied its effect on the detoxification function of the liver

Methods

Results

Conclusion