Abstract
The biosynthetic pathways of bacterial cellulose (BC) in Acetobacter xylinum were precisely examined by using culture media containing D-(l-13C)glucose, (2-13C)glucose or (6-13C)glucose as the carbon source. Quantitative analysis of the NMR spectra of the glucose hydrolyzed from synthesized BC allows us to estimate the percentage of which metabolic pathway the fed glucose pass through, such as the pentose phosphate cycle (PC) and Entner-Doudoroff (ED) pathway. The results indicated that the rate of direct polymerization (DP) of glucose intensely increased to 47% by ethanol addition compared to the standard (16%). The other pathways (PC and ED) decreased to 30% from 35%, to 8% from 41%, respectively. From these results, it is considered that the role of ethanol is to act as the energy source for proliferation of cells instead of PC or ED pathways and a large part of the glucose which does not pass through PC or ED is used for BC production through DP.
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