Abstract
Six monkeys ( Macaca sinica) were experimentally infected with Toxocara canis ova and their humoral antibody response was studied by immuno-diffusion precipitin tests using as antigens saline extracts of embryonated eggs and adult worms of T. canis and Ascaris lumbricoides respectively. In addition, saline extracts of adult worms of Ascaridia galli and Ancylostoma caninum were used to elucidate the comparative serological cross-reactivity among T. canis, A. lumbricoides, A. galli and A. caninum. The monkey sera revealed a minimum of 4 to 5 species specific antibodies and at least one group antibody specific to a common antigen component shared by T. canis and the related ascaroids, A. lumbricoides and A. galli. The specific antigens were present mainly in Toxocara embryonated egg extracts while the ascaroid group specific component was present in the Toxocara adult worm extracts. Both types of antigens differed in their electrophoretic mobilities. Fifty-three serum samples from children with larva migrans syndromes were examined for precipitins to adult and embryonated egg extracts of Ascaris lumbricoides and of Toxocara canis. A specific precipitin reaction consisting of a minimum of two overlapping bands against Toxocara egg antigen was observed in the sera of 10 children. These sera also showed an ascaroid group specific precipitin reaction consisting of a minimum of one band with the antigens of Toxocara and Ascaris. The sera of seven children showed a reaction consisting of at least one with the A. lumbricoides antigens only. Six sera showed an ascaroid group specific reaction consisting of one common band with the adult worm and egg extracts of A. lumbricoides and T. canis antigens.
Published Version
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