Abstract
This paper discusses methods to promote fungal growth and penetration in a soil contaminated with weathered crude oil. The ligninolytic enzyme activity and toxicity threshold of several white-rot fungi known for their hydrocarbon degradation ability was studied. Pine wood chips, peat moss and Kellogg's Bran Flakes were examined for their properties as the bulking agents and solid amendments. The results showed all strains developed severe toxicity at concentrations higher than 10 000 ppm. Two strains of Bjerkandera adusta UAMH 7308 and 8258 showed the highest ligninolytic enzyme activity. Furthermore, white-rot fungi did not colonize the soil without bulking agents being present in the soil. A mixture of peat moss with bran flakes resulted in the best growth, penetration and enzyme activity in the soil.
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