Removal of estrogenic activity of natural steroidal hormone estrone by ligninolytic enzymes from white rot fungi

Abstract

Natural steroidal hormone estrone (E 1) was treated with the white rot fungus Phanerochaete sordida YK-624 under ligninolytic condition with low-nitrogen and high-carbon culture medium. E 1 decreased by 98% after 5 d of treatment and the activities of ligninolytic enzymes, manganese peroxidase (MnP) and laccase, were detected during treatment, which suggested that the disappearance of E 1 is related to ligninolytic enzymes produced extracellularly by white rot fungus. Therefore, E 1 was treated with MnP and laccase prepared from the culture of white rot fungi. HPLC analysis demonstrated that E 1 disappeared completely in the reaction mixture after 1 h of treatment with either MnP or laccase. Using the yeast two-hybrid assay system, it was also confirmed that both enzymatic treatments completely removed the estrogenic activity of E 1 after 2 h. These results strongly suggest that ligninolytic enzymes are effective in removing the estrogenic activity of E 1.