Abstract
Objective To research the feasibility and clinical application value of high-risk human papilloma virus (HR-HPV) E6/E7 mRNA and DNA transcripts in cervical cancer samples by using liquid-based Thin-prep cytology test (TCT). Methods Both high-risk HPV E6/E7 mRNA and DNA of 22 samples by two different methods of Specimen handling were detected. The data from detection of high-risk HPV E6/E7 mRNA and HPV E6/E7 DNA in 108 TCT samples with different cervical lesions were statistically analyzed. Results Willcoxon rank test was used to exam two different methods of Specimen handling (P> 0.05). The expression level of HPV E6/E7 mRNA transcripts was related to the development of severe epithelial cervical dysplasia. The expression copy number of HPV E6/E7 mRNA transcripts in 108 Thin-prep cytology test was 320.554 ± 779.104. The expression copy number of HPV E6/E7 DNA transcripts in 108 Thin-prep cytology tests was 1540.963 ± 3575.255. The coefficient of Spearman test among HPV DNA and E6/E7 mRNA was 1. Conclusions The residual Thin-prep cytology test samples could be used in the detection of high-risk HPV E6/E7 mRNA and HPV E6/E7 DNA. HPV E6 and E7 mRNA test could be a useful tool for cervical cancer screening. Key words: human papilloma virus (HPV); mRNA; DNA; Thin-prep cytology test (TCT); E6/E7
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