Abstract
In this present work, a prednisolone ion selective electrode (PRED-ISE) has been developed. The electrode shows linear response towards prednisolone in the range 3.0×10−6 – 8.6×10−3 M with a detection limit of 2.5×10−6. PRED-ISE was used as an indicator electrode for the potentiometric titration of different concentrations of standard prednisolone against standardized sodium tetraphenyl borate solution and in tablets. The electrode manifests advantages of low resistance, fast response and, most importantly, good selective relativity to a variety of other cations.
Highlights
Prednisolone is a synthetic steroid that is chemically defined as 11β,17α,21-trihydroxypregna-1,4-diene-3,20-dione (Fig. 1)
Membranes plasticized with O-NPOE gave a slightly better detection limit compared with DBP, so O-NPOE was chosen for subsequent investigations
The influence of the pH of a test solution on the proposed PRED electrode potentiometric response was studied at three PRED concentrations (1x 10-4, 1x 10-3 and 1x10-2 M) where the pH was adjusted from 1 to 10 with HCl and/or NaOH (0.1–1.0 M)
Summary
Prednisolone is a synthetic steroid that is chemically defined as 11β,17α,21-trihydroxypregna-1,4-diene-3,20-dione (Fig. 1). Prednisolone is a glucocorticoid that is extensively used in clinical practice. Several methods based on liquid chromatography with UV detection have been reported [1,2,3,4,5,6,7,8,9,10,11,12], for the determination of prednisolone in plasma and some voltammetric methods have been developed [13,14,15,16]. The present method aimed at advocating of a new PRED-ISE as simple and accurate tool for the determination of prednisolone in authentic solutions, tablets and in spiked urine samples. Stirred aqueous suspension containing 0.3604 g PRED till complete dissolution of the solid and made up to 100 mL with distilled water
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