Potentiating cutaneous wound healing in young and aged skin with nutraceutical collagen peptides.

K Mistry,B Van Der Steen,P E Lovat,F Van Holthoon,J Prawitt,B Vanhoecke,M Labus,A Kleinnijenhuis,T Clifford,A Mcconnell

doi:10.1111/ced.14392

K Mistry, B Van Der Steen + Show 8 more

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https://doi.org/10.1111/ced.14392

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Abstract

Chronic wounds continue to be a burden to healthcare systems, with ageing linked to increased prevalence of chronic wound development. Nutraceutical collagen peptides have been shown to reduce signs of skin ageing, but their therapeutic potential for cutaneous wound healing remains undefined. To determine the potential for nutraceutical collagen peptides to promote cutaneous wound healing in vitro in the context of age. The potential for bovine- or porcine-derived nutraceutical collagen peptides to promote wound healing of primary cutaneous fibroblasts and keratinocytes derived from young and aged individuals in vitro was assessed by two-dimensional scratch and cell-viability assays and by immunofluorescence for the cell proliferation marker, Ki67. The achievement of peptide concentrations in vivo, equivalent to those exerting a beneficial effect on wound healing in vitro, was confirmed by pharmacokinetic studies of hydroxyproline, a biomarker for collagen peptide absorption, following peptide ingestion by healthy individuals over a wide age range. Results demonstrated significant nutraceutical collagen peptide-induced wound closure of both young and aged fibroblasts and keratinocytes, mediated by enhanced cellular proliferation and migration. Analysis of blood levels of hydroxyproline in young and aged individuals following porcine collagen peptide ingestion revealed peak serum/plasma levels after 2h at similar concentrations to those exerting beneficial effects on wound healing in vitro. These data demonstrate the capacity for nutraceutical collagen peptides to promote cutaneous wound closure in vitro, at pharmacologically achievable concentrations in vivo, thereby offering a potential novel therapeutic strategy for the management of cutaneous wounds in young and aged individuals.

Highlights

Chronic wounds are a major clinical and financial burden to worldwide healthcare systems, accountingAgeing has a negative impact on normal woundhealing responses, with age-related changes occurring in the structure and function of the extracellular matrix (ECM) as well as changes in growth factor secretion, contributing to chronic wound a 2020 The Authors
Collagen I but not collagen III expression decreases with age Analysis of collagen I and III expression by young, middle-aged or aged fibroblasts revealed a significant (*P < 0.05) decrease in collagen I expression in aged compared with young fibroblasts (Fig. 1a,b), but no significant difference in collagen III expression was observed between aged fibroblasts from different aged individuals (Fig. 1c,d)
To determine the potential for bovine or porcine nutraceutical collagen peptides to promote cutaneous wound closure in vitro, wound closure of primary fibroblasts or keratinocytes derived from young or aged individuals seeded on collagen peptide-coated plates was monitored using two-dimensional scratch assays, with 10 nmol/L CXCL12 treatment used as a positive control (*P < 0.05, **P < 0.01; Fig. S2, Data S2)

Summary

Introduction

Chronic wounds are a major clinical and financial burden to worldwide healthcare systems, accountingAgeing has a negative impact on normal woundhealing responses, with age-related changes occurring in the structure and function of the extracellular matrix (ECM) as well as changes in growth factor secretion, contributing to chronic wound a 2020 The Authors. John Wiley & Sons Ltd on behalf of British Association of Dermatologists. Development.[4,5] Aged skin displays reduced collagen synthesis and increased matrix metalloproteinase (MMP) secretion, leading to increased collagen degradation.[6,7,8] while young skin mounts a robust and timely wound-healing response, aged skin displays extended inflammatory and remodelling phases, leading to delayed wound closure, decreased wound strength and the increased incidence of medical complications associated with chronic wounds in the elderly.[5]

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