Potentially Bioaccessible Phenolics from Mung Bean and Adzuki Bean Sprouts Enriched with Probiotic-Antioxidant Properties and Effect on the Motility and Survival of AGS Human Gastric Carcinoma Cells.

Michał Świeca,Anna Herok,Ireneusz Kapusta,Katarzyna Piwowarczyk,Urszula Gawlik-Dziki,Patryk Ostanek,Jarosław Czyż,Małgorzata Sikora

doi:10.3390/molecules25132963

Michał Świeca, Anna Herok + Show 6 more

Open Access

https://doi.org/10.3390/molecules25132963

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Abstract

Gastric digests from mung (MBS) and adzuki (ABS) bean sprouts enriched with probiotic Lactobacillus plantarum 299v were tested for their antioxidant potential, as well as antiproliferative and antimotility properties, in human stomach cancer cells (AGS). The digest of ABS contained quercetin and kaempferol derivates, while kaempferol and apigenin derivates were dominant in MBS. Compared to the controls, the probiotic-rich sprouts had a higher antioxidant potential—by 13% and 9%, respectively. Adzuki bean sprouts decreased the viability of AGS already at low concentrations (25% motility inhibitions). MBS and ABS displayed dose-independent cytostatic effects. The ABS extracts decreased the proliferation of AGS more effectively than the MBS extracts—0.2‰ ABS exerted c.a. 70% of inhibitions. Moreover, the phytochemicals from the probiotic-rich sprouts considerably reduced this activity. The increased vinculin level, the apoptotic shape of cell nuclei, and the reduced cell motility and proliferation indicate that the extracts exhibited cytostatic and cytotoxic activity.

Highlights

The incidence of gastric cancer varies greatly across countries, this tumor is the fifth most common malignancy and the third leading cause of cancer-related mortality [1]
The aim of the study was to investigate the antiproliferative effects and the ability to reduce the motility of human stomach cancer cells (AGS) by fractions obtained after the gastric digestion of mung bean and adzuki bean sprouts enriched with probiotic L. plantarum 299v
A double strategy was employed for designing new functional products with improved anticancer properties, i.e., a combination of legumes with probiotic L. plantarum 299v

Summary

Introduction

The incidence of gastric cancer varies greatly across countries, this tumor is the fifth most common malignancy and the third leading cause of cancer-related mortality [1]. It is associated with environmental and genetic predisposition factors, including an unhealthy diet and lifestyle (e.g., high-salt food, smoking, and drinking). In vivo anticancer activity strongly depends on the bioaccessibility, bioavailability, and metabolic fate of phenolics They are usually absorbed through passive diffusion or mediated uptake into the intestine epithelial wall and are, metabolized by phase I and II enzymes (e.g., CYPs, UGTs, and SULTs) into various metabolites [4]. In some cases (e.g., digestive tract cancers), phenolics may be directly absorbed by cancer cells and exert side effects [5]

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